上海信帆供應(yīng)：人氧化低密度脂蛋白

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OXIDIZED LOW DENSITY LIPOPROTEIN, HUMAN

Quantity: 2.0mg (milligrams) Protein/vial

Concentration: 2.00mg/ml (Protein)

Packaging: Oxidized LDL is membrane filtered and aseptically packaged under nitrogen in a solution containing phosphate-buffered saline at pH 7.4 and 0.2mM EDTA-Na2.

Preparation: Human LDL, which was purified to homogeneity via ultracentrifugation(1.019-1.063g/cc), is oxidized using Cu2SO4 (oxidant) in PBS at 37°C for 18hours. Oxidation is terminated by adding excess EDTA-Na2. Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of OxLDL migrates 2.0fold further than the native LDL. This type Oxidized LDL is widely used to investigate lipid metabolism, only less induce cell apoptosis. Apoptosis inducement please choose High Oxidized LDL from our company.

TBARS: TBARS is determined colorimetrically by using Malondialdehyde as a standard.

Starting LDL<0.50 nmoles of MDA/mg Protein

Ox-LDL >18.5 nmoles of MDA/mg Protein

Endotoxin: < 0.5EU/mg(Protein)

Storage & Stability: This product is stable for 6 weeks after receipt when handled aseptically and stored at 2-8°C.

NEVER FREEZE.

*Special Note: After prolonged storage, some precipitate may be observed. This is normal

for lipoprotein. Spin in a centrifugation 1000×g for 3 minutes before using.

Biological Sample lots of YB Oxidized LDL are evaluated for receptor binding to

Activity: peritoneal macrophages in conjunction with our DiI-Ox-LDL and [I-125] Ox-LDL

*Preparations of Oxidized LDL are fairly unstable; plan your experiments in advance and use fresh material.

上海信帆供應(yīng)：氧化低密度脂蛋白